Journal: Obesity Facts

Article Title: The Effect of SH2B1 Variants on Expression of Leptin- and Insulin-Induced Pathways in Murine Hypothalamus

doi: 10.1159/000486962

Figure Lengend Snippet: Changes in gene expression after transfection of CLU188 cells with human SH2B1 containing variants and stimulation with leptin

Article Snippet: The hypothalamic CLU188 cells (‘Adult mouse hypothalamic cell line mHypoA-2/28 CLU188’;Biozol, Eching, Germany) were cultured in DMEM (Life Technologies, Carlsbad, CA, USA) with 10% fetal bovine serum (FBS; Merck Millipore, Darmstadt, Germany) and penicillin-streptomycin (Life Technologies, Carlsbad, CA, USA) at 37 °C in humidified atmosphere containing 5% CO 2 .

Techniques: Expressing, Transfection

Journal: Obesity Facts

Article Title: The Effect of SH2B1 Variants on Expression of Leptin- and Insulin-Induced Pathways in Murine Hypothalamus

doi: 10.1159/000486962

Figure Lengend Snippet: Genes with expression changes in hypothalamic CLU188 after stimulation with leptin. For the depicted genes, we observed nominal changes in expression (biological triplicate) for at least one of the analyzed SH2B1 variants (p ≤ 0.05). Fold change from the alpha wild-type splice form is given. *Nominal p value < 0.01, **Nominal p value < 0.001. Most severe changes in expression were detected for Gbp2b (67Cys, pnominal = 0.0002), Irf9 (α689Leu, pnominal = 0.000018), Isg15 (150Arg, pnominal = 0.0003). Gene names: cyclin D1 (Ccnd1); Fatty acid synthase (Fas); guanylate binding protein 2, interferon-inducible (Gbp2b); interleukin 20 (Il20); insulin receptor (Insr); interferon regulatory factor 1 and 9 (Irf1, Irf9); interferon-stimulated gene 15 (Isg15); Janus kinase 1 (Jak1);SMAD family member 1 (Smad1); signal transducer and activator of transcription 1 and 2 (Stat1, Stat2); STIP1 homology and U-Box containing protein 1 (Stub1).

Article Snippet: The hypothalamic CLU188 cells (‘Adult mouse hypothalamic cell line mHypoA-2/28 CLU188’;Biozol, Eching, Germany) were cultured in DMEM (Life Technologies, Carlsbad, CA, USA) with 10% fetal bovine serum (FBS; Merck Millipore, Darmstadt, Germany) and penicillin-streptomycin (Life Technologies, Carlsbad, CA, USA) at 37 °C in humidified atmosphere containing 5% CO 2 .

Techniques: Expressing, Binding Assay

Journal: Obesity Facts

Article Title: The Effect of SH2B1 Variants on Expression of Leptin- and Insulin-Induced Pathways in Murine Hypothalamus

doi: 10.1159/000486962

Figure Lengend Snippet: Changes in gene expression after transfection of CLU188 cells with human SH2B1 containing variants and stimulation with insulin

Article Snippet: The hypothalamic CLU188 cells (‘Adult mouse hypothalamic cell line mHypoA-2/28 CLU188’;Biozol, Eching, Germany) were cultured in DMEM (Life Technologies, Carlsbad, CA, USA) with 10% fetal bovine serum (FBS; Merck Millipore, Darmstadt, Germany) and penicillin-streptomycin (Life Technologies, Carlsbad, CA, USA) at 37 °C in humidified atmosphere containing 5% CO 2 .

Techniques: Expressing, Transfection

Journal: Obesity Facts

Article Title: The Effect of SH2B1 Variants on Expression of Leptin- and Insulin-Induced Pathways in Murine Hypothalamus

doi: 10.1159/000486962

Figure Lengend Snippet: Genes with expression changes in hypothalamic CLU188 after stimulation with insulin. For the depicted genes, we observed nominal changes in expression (biological triplicate) for at least one of the analyzed SH2B1 variants. Fold change from the alpha wild-type splice form is given. *Nominal p value < 0.01. Gene names: cyclase-associated protein 1 (Cap1); mitogen-activated protein kinase 1 (Mapk1); sorbin and SH3 domain containing 1 (Sorbs1).

Article Snippet: The hypothalamic CLU188 cells (‘Adult mouse hypothalamic cell line mHypoA-2/28 CLU188’;Biozol, Eching, Germany) were cultured in DMEM (Life Technologies, Carlsbad, CA, USA) with 10% fetal bovine serum (FBS; Merck Millipore, Darmstadt, Germany) and penicillin-streptomycin (Life Technologies, Carlsbad, CA, USA) at 37 °C in humidified atmosphere containing 5% CO 2 .

Techniques: Expressing